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biotinylated goat anti mouse ccl22  (R&D Systems)


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    R&D Systems biotinylated goat anti mouse ccl22
    Biotinylated Goat Anti Mouse Ccl22, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated goat anti mouse ccl22/product/R&D Systems
    Average 90 stars, based on 4 article reviews
    biotinylated goat anti mouse ccl22 - by Bioz Stars, 2026-03
    90/100 stars

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    Peptide immunotherapy inhibits levels of Th2 cytokines, Th2 chemokines, and serum IgE levels. Mice were sacrificed at day 61. Serum was obtained by cardiac puncture, BAL was performed to obtain BAL fluid, and single lung lobes were harvested and enzymatically digested. (a) BAL fluid and (b) lung digest homogenate cytokines IL-4, IL-5, IL-13, and IFN-γ were measured by ELISA according to manufacturer’s protocols. Th2 chemokines CCL11/eotaxin, CCL17/TARC, and CCL22/MDC in BAL fluid (c) and lung digest homogenates (d) were measured by ELISA according to the manufacturer’s protocols. Total IgE in serum samples (e) was quantified by ELISA using paired antibodies according to the manufacturer’s protocol. Levels of Fel d 1–specific IgE (f) were measured in serum by coating plates with recombinant Fel d 1 (5 µg/ml), incubating with serum samples, and detecting bound IgE with chromogen-labeled anti-IgE. Data are expressed as mean ± SEM. n = 5 mice/group, and are representative of three independent experiments. *, P < 0.05 and **, P < 0.01 for sensitized/HA (306–318) compared with sensitized/DR1 Feld1 -treated mice.

    Journal: The Journal of Experimental Medicine

    Article Title: Peptide immunotherapy in allergic asthma generates IL-10–dependent immunological tolerance associated with linked epitope suppression

    doi: 10.1084/jem.20082901

    Figure Lengend Snippet: Peptide immunotherapy inhibits levels of Th2 cytokines, Th2 chemokines, and serum IgE levels. Mice were sacrificed at day 61. Serum was obtained by cardiac puncture, BAL was performed to obtain BAL fluid, and single lung lobes were harvested and enzymatically digested. (a) BAL fluid and (b) lung digest homogenate cytokines IL-4, IL-5, IL-13, and IFN-γ were measured by ELISA according to manufacturer’s protocols. Th2 chemokines CCL11/eotaxin, CCL17/TARC, and CCL22/MDC in BAL fluid (c) and lung digest homogenates (d) were measured by ELISA according to the manufacturer’s protocols. Total IgE in serum samples (e) was quantified by ELISA using paired antibodies according to the manufacturer’s protocol. Levels of Fel d 1–specific IgE (f) were measured in serum by coating plates with recombinant Fel d 1 (5 µg/ml), incubating with serum samples, and detecting bound IgE with chromogen-labeled anti-IgE. Data are expressed as mean ± SEM. n = 5 mice/group, and are representative of three independent experiments. *, P < 0.05 and **, P < 0.01 for sensitized/HA (306–318) compared with sensitized/DR1 Feld1 -treated mice.

    Article Snippet: CCL22 levels were measured using a sandwich ELISA generated by coating ELISA plates with anti–mouse MDC (a gift from ICOS Corp.) and detecting bound antibody with biotinylated anti–mouse MDC (R&D Systems) against a standard curve generated using recombinant MDC (R&D Systems) as previously described ( ).

    Techniques: Enzyme-linked Immunosorbent Assay, Recombinant, Labeling